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umbilical vascular endothelial cells huvecs  (ATCC)


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    ATCC umbilical vascular endothelial cells huvecs
    Umbilical Vascular Endothelial Cells Huvecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/umbilical+vascular+endothelial+cells+huvecs/Primary+Umbilical+Vein+Endothelial+Cells%3B+Normal%2C+Human/pm41108330-41-23-28
    Average 99 stars, based on 4947 article reviews
    umbilical vascular endothelial cells huvecs - by Bioz Stars, 2026-10
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    Related Articles

    Gene Expression:

    Article Title: SVEP1 as a Genetic Modifier of TEK -Related Primary Congenital Glaucoma
    Article Snippet: Cell localization was studied 24 hours after transfection on glass cover slips using an anti-FLAG-FITC antibody (1:500; M2, F4049, Sigma-Aldrich Corp.), ProLong Diamond antifade mountant with DAPI (Thermo Fisher Scientific), and an Olympus BX51 fluorescence microscope. .. The ability of extracellular SVEP1 to stimulate TEK gene expression in human umbilical vascular endothelial cells (HUVECs) (PCS-100-013; ATCC, Manassas, VA) was assessed as follows: WT-SVEP1, p.R997C-SVEP1, and empty FLAG vector were expressed by HEK293T cells in vascular cell basal medium (VCBM; PCS-100-030; ATCC) supplemented with Endothelial Cell Growth Kit-VEGF (PCS-100-041; ATCC) for 2 days. .. Cell-free conditioned media was collected, added to growing HUVECs for 24 hours, the cells lysed and processed using a Cells-to-cDNA II kit (Thermo Fisher Scientific), and quantitative real-time PCR performed to assess TEK gene expression (TaqMan probe Hs00176096_m1, FAM-MGB; Thermo Fisher Scientific).

    Plasmid Preparation:

    Article Title: SVEP1 as a Genetic Modifier of TEK -Related Primary Congenital Glaucoma
    Article Snippet: Cell localization was studied 24 hours after transfection on glass cover slips using an anti-FLAG-FITC antibody (1:500; M2, F4049, Sigma-Aldrich Corp.), ProLong Diamond antifade mountant with DAPI (Thermo Fisher Scientific), and an Olympus BX51 fluorescence microscope. .. The ability of extracellular SVEP1 to stimulate TEK gene expression in human umbilical vascular endothelial cells (HUVECs) (PCS-100-013; ATCC, Manassas, VA) was assessed as follows: WT-SVEP1, p.R997C-SVEP1, and empty FLAG vector were expressed by HEK293T cells in vascular cell basal medium (VCBM; PCS-100-030; ATCC) supplemented with Endothelial Cell Growth Kit-VEGF (PCS-100-041; ATCC) for 2 days. .. Cell-free conditioned media was collected, added to growing HUVECs for 24 hours, the cells lysed and processed using a Cells-to-cDNA II kit (Thermo Fisher Scientific), and quantitative real-time PCR performed to assess TEK gene expression (TaqMan probe Hs00176096_m1, FAM-MGB; Thermo Fisher Scientific).

    Multiple Displacement Amplification:

    Article Title: Synergistic combination of the adrenergic antagonist SR59230A with common chemotherapeutic drugs and target therapies in cancer and endothelial cells.
    Article Snippet: β3-adrenergic receptors (β3-ARs) are increasingly recognized as modulators of tumor progression and treatment resistance across multiple cancer types.. SR59230A, a β3-AR antagonist, has shown preclinical antitumor activity through mechanisms involving mitochondrial reactivation, reactive oxygen species (ROS) production, and antiangiogenic effects.. Based on this premise, this study aimed to investigate the in vitro synergistic effects of SR59230A combined with standard chemotherapeutics or targeted therapies in various human cancer cell lines (glioblastoma, melanoma, triple-negative breast cancer, and anaplastic thyroid carcinoma) and endothelial cells (HUVECs).

    Cell Culture:

    Article Title: How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells?
    Article Snippet: .. 2.7 Human umbilical vascular endothelial cells (HUVECs), mesenchymal stem cells (MSCs) and granulocytic cultures Primary HUVECs were obtained from ATCC and cultured according to ATCC recommendation (growth medium: vascular cell basal medium (ATCC) supplemented with endothelial cell growth kit-VEGF (ATCC)). ..

    Article Title: Silver nanoparticles modify VEGF signaling pathway and mucus hypersecretion in allergic airway inflammation
    Article Snippet: .. Cell culture The human umbilical vascular endothelial cells (HUVECs) were purchased from American Type Culture Collection (Rockville, MD). .. HUVECs were cultured in cell growth medium-2 (Lonza, Walkersville, MD) per manufacturer’s recommendations on 2% gelatin-coated dishes.



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    ( A ) Membrane labeling of <t>HUVEC</t> monolayers with CellMask. ( B ) Corresponding fluorescent bead image (beads of size 0.2 μ m ). Arrows indicate bead-related image artifacts ( C ) PIV-UQ displacement field u PIV ( D ) PIV-UQ uncertainty map, σ u , P I V . White regions indicate “bad” PIV windows that were deleted and replaced as described in § 2.2. Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of bootstrapped PIV-UQ distribution. ( E ) Inferred mean marginal posterior traction stress, t ^ ( F ) Marginal posterior traction stress uncertainty field ( σ t ). Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of marginal posterior p ( t | u h ) . Scale bar : 25 μ m
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    ( A ) Membrane labeling of <t>HUVEC</t> monolayers with CellMask. ( B ) Corresponding fluorescent bead image (beads of size 0.2 μ m ). Arrows indicate bead-related image artifacts ( C ) PIV-UQ displacement field u PIV ( D ) PIV-UQ uncertainty map, σ u , P I V . White regions indicate “bad” PIV windows that were deleted and replaced as described in § 2.2. Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of bootstrapped PIV-UQ distribution. ( E ) Inferred mean marginal posterior traction stress, t ^ ( F ) Marginal posterior traction stress uncertainty field ( σ t ). Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of marginal posterior p ( t | u h ) . Scale bar : 25 μ m
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    Image Search Results


    ( A ) Membrane labeling of HUVEC monolayers with CellMask. ( B ) Corresponding fluorescent bead image (beads of size 0.2 μ m ). Arrows indicate bead-related image artifacts ( C ) PIV-UQ displacement field u PIV ( D ) PIV-UQ uncertainty map, σ u , P I V . White regions indicate “bad” PIV windows that were deleted and replaced as described in § 2.2. Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of bootstrapped PIV-UQ distribution. ( E ) Inferred mean marginal posterior traction stress, t ^ ( F ) Marginal posterior traction stress uncertainty field ( σ t ). Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of marginal posterior p ( t | u h ) . Scale bar : 25 μ m

    Journal: PLOS Computational Biology

    Article Title: Uncertainty-aware traction force microscopy

    doi: 10.1371/journal.pcbi.1013079

    Figure Lengend Snippet: ( A ) Membrane labeling of HUVEC monolayers with CellMask. ( B ) Corresponding fluorescent bead image (beads of size 0.2 μ m ). Arrows indicate bead-related image artifacts ( C ) PIV-UQ displacement field u PIV ( D ) PIV-UQ uncertainty map, σ u , P I V . White regions indicate “bad” PIV windows that were deleted and replaced as described in § 2.2. Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of bootstrapped PIV-UQ distribution. ( E ) Inferred mean marginal posterior traction stress, t ^ ( F ) Marginal posterior traction stress uncertainty field ( σ t ). Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of marginal posterior p ( t | u h ) . Scale bar : 25 μ m

    Article Snippet: Human vascular umbilical endothelial vein cells (HUVECs) (Cell Applications) were cultured in M199 (Gibco) supplemented with 10 % (v/v) endothelial growth medium (Cell Applications), 10 % (v/v) fetal bovine serum (Gibco), and 1 % penicillin-streptomycin (Gibco).

    Techniques: Membrane, Labeling

    Preparation, characterization, ROS scavenging, and biocompatibility of VPNS@P (A) Schematic of exfoliation and PEGylation of violet phosphorus (VP) to prepare VPNS@P from bulk VP. (B) Transmission electron microscopy (TEM) image of VPNS@P. Scale bar, 100 nm. (C) Hydrodynamic size distribution of VPNS@P measured by dynamic light scattering (DLS). (D and E) Atomic force microscopy (AFM) image (D) and thickness profile (E) of VPNS@P. Scale bar, 100 nm. (F) Raman scattering spectra of VPNS@P. (G) Time-course DLS measurements of VPNS@P incubated in PBS or DMEM supplemented with 10% fetal bovine serum (FBS) over 7 days ( n = 3 independent samples). (H–J) Scavenging capability of VPNS@P ( n = 5 independent samples) toward H 2 O 2 (H), ·OH (I), and O 2 ·− (J). (K–M) Biocompatibility of VPNS@P in vitro . Cell viabilities of RAW264.7 (K), mouse aortic vascular smooth muscle cells (MOVASs) (L), and human umbilical vein endothelial cells (HUVECs) (M) were examined with a CCK-8 assay ( n = 3 biologically independent samples). Data were analyzed using one-way ANOVA with a Dunnett’s T3 post hoc test and are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. ns, not significant.

    Journal: Cell Reports Medicine

    Article Title: Restoring immune homeostasis in atherosclerotic plaques via inorganic violet phosphorus nano-immunotherapy

    doi: 10.1016/j.xcrm.2025.102528

    Figure Lengend Snippet: Preparation, characterization, ROS scavenging, and biocompatibility of VPNS@P (A) Schematic of exfoliation and PEGylation of violet phosphorus (VP) to prepare VPNS@P from bulk VP. (B) Transmission electron microscopy (TEM) image of VPNS@P. Scale bar, 100 nm. (C) Hydrodynamic size distribution of VPNS@P measured by dynamic light scattering (DLS). (D and E) Atomic force microscopy (AFM) image (D) and thickness profile (E) of VPNS@P. Scale bar, 100 nm. (F) Raman scattering spectra of VPNS@P. (G) Time-course DLS measurements of VPNS@P incubated in PBS or DMEM supplemented with 10% fetal bovine serum (FBS) over 7 days ( n = 3 independent samples). (H–J) Scavenging capability of VPNS@P ( n = 5 independent samples) toward H 2 O 2 (H), ·OH (I), and O 2 ·− (J). (K–M) Biocompatibility of VPNS@P in vitro . Cell viabilities of RAW264.7 (K), mouse aortic vascular smooth muscle cells (MOVASs) (L), and human umbilical vein endothelial cells (HUVECs) (M) were examined with a CCK-8 assay ( n = 3 biologically independent samples). Data were analyzed using one-way ANOVA with a Dunnett’s T3 post hoc test and are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. ns, not significant.

    Article Snippet: Human: HUVEC umbilical vein endothelial cells , ATCC , CRL-1730; RRID: CVCL_2959.

    Techniques: Transmission Assay, Electron Microscopy, Microscopy, Incubation, In Vitro, CCK-8 Assay