umbilical vascular endothelial cells huvecs  (ATCC)

Journal: PLOS Computational Biology

Article Title: Uncertainty-aware traction force microscopy

doi: 10.1371/journal.pcbi.1013079

Figure Lengend Snippet: ( A ) Membrane labeling of HUVEC monolayers with CellMask. ( B ) Corresponding fluorescent bead image (beads of size 0.2 μ m ). Arrows indicate bead-related image artifacts ( C ) PIV-UQ displacement field u PIV ( D ) PIV-UQ uncertainty map, σ u , P I V . White regions indicate “bad” PIV windows that were deleted and replaced as described in § 2.2. Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of bootstrapped PIV-UQ distribution. ( E ) Inferred mean marginal posterior traction stress, t ^ ( F ) Marginal posterior traction stress uncertainty field ( σ t ). Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of marginal posterior p ( t | u h ) . Scale bar : 25 μ m

Article Snippet: Human vascular umbilical endothelial vein cells (HUVECs) (Cell Applications) were cultured in M199 (Gibco) supplemented with 10 % (v/v) endothelial growth medium (Cell Applications), 10 % (v/v) fetal bovine serum (Gibco), and 1 % penicillin-streptomycin (Gibco).

Techniques: Membrane, Labeling

Journal: Cell Reports Medicine

Article Title: Restoring immune homeostasis in atherosclerotic plaques via inorganic violet phosphorus nano-immunotherapy

doi: 10.1016/j.xcrm.2025.102528

Figure Lengend Snippet: Preparation, characterization, ROS scavenging, and biocompatibility of VPNS@P (A) Schematic of exfoliation and PEGylation of violet phosphorus (VP) to prepare VPNS@P from bulk VP. (B) Transmission electron microscopy (TEM) image of VPNS@P. Scale bar, 100 nm. (C) Hydrodynamic size distribution of VPNS@P measured by dynamic light scattering (DLS). (D and E) Atomic force microscopy (AFM) image (D) and thickness profile (E) of VPNS@P. Scale bar, 100 nm. (F) Raman scattering spectra of VPNS@P. (G) Time-course DLS measurements of VPNS@P incubated in PBS or DMEM supplemented with 10% fetal bovine serum (FBS) over 7 days ( n = 3 independent samples). (H–J) Scavenging capability of VPNS@P ( n = 5 independent samples) toward H 2 O 2 (H), ·OH (I), and O 2 ·− (J). (K–M) Biocompatibility of VPNS@P in vitro . Cell viabilities of RAW264.7 (K), mouse aortic vascular smooth muscle cells (MOVASs) (L), and human umbilical vein endothelial cells (HUVECs) (M) were examined with a CCK-8 assay ( n = 3 biologically independent samples). Data were analyzed using one-way ANOVA with a Dunnett’s T3 post hoc test and are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. ns, not significant.

Article Snippet: Human: HUVEC umbilical vein endothelial cells , ATCC , CRL-1730; RRID: CVCL_2959.

Techniques: Transmission Assay, Electron Microscopy, Microscopy, Incubation, In Vitro, CCK-8 Assay